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Aman Patel, Reetesh Yadav*, Deepak Patel
Shri Ram Institute of Pharmacy, Jabalpur 482002 MP, India
*Address for Corresponding author
Dr. Reetesh Yadav
Shri Ram Institute of Pharmacy, Jabalpur 482002 MP, India
Abstract
Background: The fast proliferation of keratinocytes is a defining feature of psoriasis, a chronic inflammatory skin disease. For this illness to be managed, the development of potent therapeutic treatments is essential. Objective: Isolation, characterization and in-silico investigation of lawsone adme for the treatment of psoriasis using in-vitro MTT keratinocyte cell line; HaCaT method. Materials and methods: Lawsone, a naturally occurring substance obtained from Lawsonia inermis, was identified, examined, and its potential as a psoriasis treatment assessed in this work. Using the HaCaT keratinocyte cell line, the in-vitro MTT test was used to evaluate the compound's effectiveness. Solvent extraction and chromatographic procedures were utilized for the isolation and purification of lawsone. Utilizing spectroscopic techniques, the compound's structure was determined. The MTT assay was utilized to assess the cytotoxicity of lawsone on HaCaT cells in vitro. This assay gauges the vitality and proliferation of cells. The outcomes showed a dose-dependent decrease in cell viability, suggesting lawsone may have an anti-proliferative effect on keratinocytes. Results: Moreover, lawsone's pharmacokinetic characteristics were predicted using in-silico ADME (Absorption, Distribution, Metabolism, and Excretion) study. According to the computational studies, lawsone has good oral bioavailability, moderate metabolism, and low toxicity, all of which are advantageous ADME properties. These results provide credence to the possibility of lawsone being investigated further as a psoriasis treatment agent. Conclusion: Its anti-proliferative action on keratinocytes and advantageous pharmacokinetic features demonstrate lawsone's potential benefits in the treatment of psoriasis, as this study demonstrates. To ensure its safety and efficacy in people, more in vivo research and clinical trials are suggested.
Keywords: Psoriasis, in-silico investigation, lawsone, HaCaT cell, MTT assay
