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Vinay Bharti, Atul Kumar Gaun, Santram Lodhi*
Sri Sathya Sai Institute of Pharmaceutical Sciences, RKDF University, Bhopal (M.P.) 462033
*Address for Corresponding Author
Dr. Santram Lodhi
Sri Sathya Sai Institute of Pharmaceutical Sciences, RKDF University, Bhopal (M.P.) 462033
Abstract
Objective: Wound healing process consists of different phases such as granulation, collagen maturation and scar maturation are some of the many phases, which are independent to each other. Present study was aimed to investigate wound healing effect of Helicteres isora roots in diabetic animals. Material and methods: Ethanolic extract of Helicteres isora seeds was selected for 5%w/w ointment preparation using simple ointment base B.P. Ethanol extract ointment (5%w/w) was evaluated for wound healing activity using diabetic wound model. Healing effect was compared with Povidone iodine ointment. Healing effect was observed by contraction area and assessment of various biochemical parameters e.g. hydroxyproline measurement, protein estimation, and histological study. Results and conclusion: From 10 to 20 days, in group treatment with 5% w/w EEHI ointment was observed significant difference in percentage of wound contraction when compared to control group. The hydroxyproline content in animals treated with EEHI Ointment (5%w/w) and reference ointment was found significantly (P<0.01) greater than control group of animals. Over the 20-day treatment period, the protein content of wounds treatment groups with EEHI Ointment (5%w/w) (72.62±3.42) and reference ointment was found significantly (P< 0.01) increased when compared to control group of animals. The activity of antioxidant enzymes was increased in EEHI Ointment (5%w/w) treated animals when compared with reference group. In the group treated with EEHI Ointment, formation of dense fibrous tissue and blood capillaries was observed on 20 days. The arrangement of capillaries was perpendicular to the fibrous tissue. Mature connective tissue and a few capillaries with a thick epidermal lining at the periphery of the wound were observed at 20 day. In conclusion, Ethanolic extract of Helicteres isora seeds contains flavonoids that were reported to have potent antioxidant and inflammatory activity.
Keywords: Helicteres isora, wound healing, diabetic, ointment, Povidone iodine ointment
