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Ahmed Ali Mustafa1*, Mubarak Siddig Hamad2, Haifa A. A. Omer3, Afaf R. Taher4
1Department of Botany and Microbiology, Faculty of Science, University of Gezira, Sudan
2Department of Taxonomy and Phytochemistry, Medicinal, Aromatic and Tradition Medicine Research Institute, National Center for Research, Khartoum, Sudan
3Department of Botany, Faculty of Science, Sudan University of Science and Technology, Sudan
4Department of Botany, Faculty of Science, Benghazi University, Benghazi, Libya
*Address for Corresponding author
Ahmed Ali Mustafa
Department of Botany and Microbiology, Faculty of Science, University of Gezira, Sudan
Abstract
The aim of this study was to evaluate the antimicrobial, antioxidant activities and quantitative determination of total polyphenol, flavonoids and tannins contents of four extracts from Chrozophora plicata and Croton zambisicus plants. Extracts from plants were prepared by sequential maceration. The antimicrobial activity was evaluated against Gram positive and Gram negative as well as two fungi by disc diffusion method. Antioxidant activity was assessed based on the scavenging activity of the stable 2,2-Diphenyl- 1-picrylhydrazyl free radical (DPPH). Total polyphenolic, flavonoids and tannins contents were determined by spectrophotometric assays. Generally, the highest results of antibacterial activity showed in methanolic extract and C. plicata., against Bacillus subtilis and Staphylococcus aureus with (inhibition zone (IZ) = 17mm) respectively. While, the highest results of antifungal activity against Candida albicans., was recorded in the methanolic extract of C. plicata and C. zambicicus (IZ) =20mm), and ethyl acetate extract of C. zambicicus gave the best activity (IZ =22mm) against Candida albicans., from the disc diffusion method. The highest scavenging radical activity was obtained from the methanol extracts of C. plicata (90%), C. zambicicus (88%) and ethyl acetate of C. plicata (88%). Quantitative analysis revealed that the total polyphenolic content was highest value showed in methanolic extract of C. plicata (157.66mg gallic acid equivalents/g). The total flavonoids content was highest value recorded from the methanolic extract of C. plicata (865.31mg quercetin equivalents/g), while, highest value of total tannins content was found in the methanol extract of C. plicata (451.92mg tannic acid equivalents/g).
Keywords: Chrozophora plicata, Croton zambisicus, total phenolic, antimicrobial, antioxidant
