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Sangeeta Saraswat, Suresh Kumar Dev*
Pacific College of Pharmacy, Pacific Academy of Higher Education and University, Udaipur, Rajasthan, India
*Address for Corresponding Author
Dr. Suresh K. Dev
Pacific College of Pharmacy, Pacific Academy of Higher Education and University, Udaipur, Rajasthan, India
Abstract
Objectives: The present study was designed to assess the preliminary phytochemical, antioxidant and antimicrobial activity of Plumbago auriculata Linn methanol extract (PAME) stem part. Material and Methods: The preliminary, phytochemical analysis, antioxidant, isolation of phenolic compound and antimicrobial activity of PAME was determined. Antioxidant activity was estimated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay method. While antimicrobial activity was determined by agar plate diffusion method using Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa bacterial and fungal strains. The isolation of phenolic compound was done by TLC method, and was characterized by UV, FTIR, and NMR spectra peaks. Results: The outcomes demonstrated that PAME found as most potential due to presence of higher phenolic content (TPC) 8.34±0.68 mg of equivalent gallic acid, measured by the Folin-ciocaltechu method. The flavonoid content (TFC) was found to be 12.7±0.81 quercetin equivalent measured by aluminium chloride colorimetric method. DPPH Free Radical Scavenging Activity showed by IC50 value was found to be 121µg/mL for the methanol extract. The antimicrobial activity was also confirmed that the PAME led to a higher inhibition zone against bacterial and fungal strains. Spectral results of UV, FTIR and NMR indicated that isolated compound was rutin. The Rutin content in the PAME was found to be 3.55 ±1.55%, w/w. Conclusion:This study suggests that the PAME from stem has strong antioxidant, TPC and TFC potential components and it could be a significant source of natural antioxidant and antimicrobial agent for topical formulation.
Keywords: Plumbago auriculatalam, antioxidant, antimicrobial, total phenolic content, total flavonoid content
