Research Articles

2022  |  Vol: 7(3)  |  Issue: 3(May-June) | https://doi.org/10.31024/apj.2022.7.3.5

Evaluation of Mearnsitrin on cardiomyocyte apoptosis induced by doxorubicin in H9c2 Cardiomyoblast Cells

Suresh Janadri*

Department of Pharmacology, Acharya & BM Reddy College of Pharmacy, Bengaluru-560090 India

^*Address for Corresponding Author

Dr. Suresh Janadri

Department of Pharmacology,

Acharya & BM Reddy College of Pharmacy,

Acharya Dr. Sarvepalli Radhakrishna Road, Bengaluru-560090, Karnataka, India

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Abstract

Objective: Doxorubicin (Dox) is a type of chemotherapy drug it slows or stops the growth of cancer cells by blocking an enzyme called topoisomerase-2 and produces cardiotoxicity by inducing apoptosis. Mearnsitrin is a flavonoid compound from the stems of Emilia sonchifolia DC and is known to be a natural antioxidant. The study is carried out the evaluation of mearnsitrin on cardiomyocyte apoptosis induced by doxorubicin in H9c2 Cardiomyoblast Cells. Materials and methods: Rat cardiac H9C2 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 100 U/ml of penicillin, 100 μg/ml of streptomycin and 5% CO2 at 37 °C.  A modified MTT assay was used to determine cell viability. Quantitative real time RT-PCR was used to evaluate the expression of Bcl-2 in cardiomyocytes. Results: No toxicity observed when the cells exposed for 1 hour to different concentrations of mearnsitrin, but pretreatment of cells with mearnsitrin increased cytotoxicity of DOX in a dose dependent manner. RT-PCR analysis showed that mearnsitrin significant mRNA gene expression of Bcl2 compared to cells treated with DOX alone. Conclusion: The data indicated that subtoxic concentrations of mearnsitrin sensitize H9c2 cells to DOX-induce apoptosis. These results suggest that the use of mearnsitrin in combination with DOX reduces the cardiomyocyte apoptosis induced in H9c2 cardiomyoblast cells than DOX alone.

Keywords: Mearnsitrin, apoptosis, doxorubicin, cardiomyoblast Cells, Emilia sonchifolia