Research Articles

2020  |  Vol: 5(3)  |  Issue: 3 (May- June) | https://doi.org/10.31024/apj.2020.5.3.3
Phytochemical investigation, antioxidant and antibacterial activities, and GC-MS analysis of ethanol extract of Terminalia chebula Retz. dried seeds

Saraswathi Krishna1, Priyadarshini2, Arumugam Perumal3*

1Karpaga Vinayaga College of Engineering and Technology, Madhuranthagam, Kancheepuram-603 308

2Meenakshi College for Women, Kodambakkam, Chennai-600 024 Tamil Nadu, India

3*ARMATS Biotek Training and Research Institute, Maduvinkarai, Guindy, Chennai-600 032 Tamil Nadu, India

*Address for Corresponding author

Dr. P. Arumugam

Industrial Fermentation Technology Division

ARMATS Biotek Training and Research Institute, Chennai-600 032, Tamil Nadu, India

 

Abstract

Objective: Terminalia chebula Retz. belongs to the family Combretaceae and it is an important potent medicinal plant used in four categories of medicines such as Ayurveda, Unani, Siddha and Homeopathy. The aim of the present study was to evaluate the antioxidant, antibacterial and anti-diabetic activities of ethanolic dried seed extract of Terminalia chebulaMaterials and Methods: In-vitro antioxidant activities such as Free radical scavenging activity by DPPH˙ method, hydroxyl (OH˙) radical scavenging, phosphomolybdenum and ferric reducing power activities respectively were performed for the selected source. Phytochemical screening was evaluated based on standardized methods. Also, the antibacterial studies were carried out by Agar well diffusion method in order to study the inhibition effect of ethanolic seed extract against the test bacterial pathogens. Detection of various phyto-constituents were been identified by Gas Chromatography-Mass Spectrometry. Results: The IC50 of DPPH˙ radical scavenging activity was 15.56µg/mL concentration respectively. Also, the RC50 of phosphomolybdenum reduction and ferric reducing power activities were 29.59 and 20.69µg/mL concentration respectively. Total phenolic and flavonoid content were determined, in which phenols were found to be significantly higher as 48.52±0.36 GAE/mg. The antibacterial activity was effective for ethanolic seed extract by agar well diffusion method, in which the maximum inhibition was against P. vulgaris and M. luteus (20 mm). The active compounds were identified by GC-MS analysis which revealed the presence of Dodecahydropyrido (1,2-b) isoquinolin-6-one, Corynan-17-ol, 18, 19-didehydro-10-methoxy-acetate (ester), Oleic acid. Conclusion: The results prove that Terminalia chebula seeds as a potent antioxidant and antibacterial agent. Hence the seeds of Terminalia chebula can be formulated as drug and also shall be tested for treating several free radicals related diseases as well as microbial infections in future.

Keywords: Terminalia chebula, Dot-plot, DPPH˙ radical, Hydroxyl radical (OH), RC50, alpha amylase

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