Research Articles

2020  |  Vol: 5(3)  |  Issue: 3 (May- June) | https://doi.org/10.31024/apj.2020.5.3.5
Preliminary pharmacological screening of Bryonia laciniosa L. for ulcerative colitis in rats

Nishkarsh Tripathi, Alok Pal Jain*

RKDF College of Pharmacy, SRK University, Hoshangabad Road, Misrod, Bhopal - 462026 (MP) India

*Address for Corresponding Author

Dr. Alok Pal Jain

RKDF College of Pharmacy, SRK University, Hoshangabad Road, Misrod, Bhopal - 462026 (MP) India

 

Abstract

Objective: Objective of present study was to investigate protective effect of ethanolic extract of Bryonia laciniosa seeds for ulcerative colitis in rats. Material and methods: Ethanolic extract of Bryonia laciniosa seeds was tested for protective effect in Dextran Sulphate Sodium (DSS) induced colitis in rats. The disease activity index and wet colon weight for different groups were observed. Treatment groups also observed for MPO activity and antioxidant substance level. Results and conclusion: DSS induced colitis significantly elevated MPO activity, whereas administration of EEBL strongly inhibited MPO activity in rats with 200mg/kg as well as near to the standard drug. The results of MDA level in tissue also indicated that colonic content of MDA decreased significantly and similar to the standard drug when compared to the DSS model group. The effect of EEBL on the various antioxidant level (SOD, CAT and GSH) were observed. The ethanolic extract of Bryonia laciniosa (EEBL) seeds restored up to the normal level of antioxidant parameters, that was confirmed the potent antioxidant effect of ethanolic extract. Phytochemical study revealed that flavonoids and phenolic compounds are present in ethanolic extract of Bryonia laciniosa seeds. The free radical scavenging property of these flavonoids plays a significant role in ulcer healing. Significant increase in GSH level and reduction in MDA level has also been revealed in extracts treated groups while investigating in vivo antioxidant activity. Hence, the probable mechanism of healing of ulcerative colitis by ethanolic extract of Bryonia laciniosa (EEBL) seeds may be attributed to antioxidant and free radical scavenging property.

Keywords: Bryonia laciniosa, Dextran Sulphate Sodium, ulcerative colitis, antioxidant

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