Dileepkumar H. V.a*, Ritesh K. R.b
aNeurobiology Lab, Department of Zoology, University of Mysore, Manasagangotri, Mysore-570006, Karnataka, India
bDepartment of Food Protectants and Infestation Control, CSIR-Central Food Technological Research Institute, Mysore-570020, Karnataka, India
*Address for Corresponding author
Dileepkumar H. V.
Department of Zoology, University of Mysore, Manasagangotri, Mysore– 570 006 Karnataka, India
Objective: Dichlorvos (DDVP) is an organophosphate insecticide used against a broad spectrum of insects and also used antihelminthic agent. It is highly toxic to mammals by inhalation, dermal absorption and ingestion. Although its neurotoxic action mediated by acetylcholine esterase inhibition is well known, the role of oxidative stress is not known. We have investigated the oxidative changes in both brain and liver on exposure to a single oral dose of DDVP in the Wistar rats. Materials and Methods: Neuro and hepatotoxic effects of DDVP was investigated in the male Wistar rats. Oxidative stress markers (reactive oxygen species, lipid peroxidation, protein carbonyls and glutathione), and antioxidant enzymes, superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductatse were measured in rats treated with single dose of DDVP (47mg/kg bw). Results: Inhibition of reactive oxygen species (ROS), lipid peroxidation (LPO) protein carbonyls (PC) and drastic depletion of glutathione (GSH) was seen in all the brain regions and the liver of DDVP treated rats. A severe reduction in the activity of superoxide dismutase (SOD) was seen in both brain regions and liver. Activities of glutathione peroxidase (GPx), glutathione reductase (GR) and catalase were also markedly decreased in the both brain regions and liver. Conclusion: DDVP showed neuro and hepatotoxicity as evident from biochemical markers. Our results clearly show that DDVP causes oxidative perturbances in the brain regions more than in the liver, which should be considered in understanding its toxic consequences.
Keywords: Dichlorvos, LPO, ROS, GSH, hepatotoxicity, neurotoxicity, oxidative stress, antioxidant enzymes