Research Articles

2019  |  Vol: 4(6)  |  Issue: 6(November-December) | https://doi.org/10.31024/apj.2019.4.6.4
Preliminary pharmacological investigations on Lens culinaris seeds for wound healing potential in rats

Atul Kumar Singh1, Santram Lodhi2, Alok Pal Jain1*

1RKDF College of Pharmacy, Savpalli Radha Krishnan University, Misrod, Bhopal (MP) 482047 India

2Sri Sathya Sai Institute of Pharmaceutical Sciences, RKDF University, Bhopal, (M.P.), India

*Address for Corresponding Author

Dr. Alok Pal Jain

RKDF College of Pharmacy, Savpalli Radha Krishnan University, Misrod, Bhopal (MP) 482047 India


Abstract

Objective: Lens culinaris Med. (family: Fabaceae) is commonly known as “lentils”. It is distributed and cultivated throughout the North region of India. Seeds are rich source of minerals such as calcium, iron, vitamin B and an important component of variety of food in many countries. The aim of present study was study preliminary phytochemical screening and pharmacological screening of Lens culinaris seeds extract for wound healing activity. Material and methods: The phytochemical analysis of ethanol extract was done for the detection of presence of different phytochemical constituents. Preliminary wound healing activity of ethanol extracts was tested by using excision wound model. Healing effect of ethanol extract of Lens culinaris seeds was investigated by measurement of percent contraction area, hydroxyproline estimation and epithelialization period. Results: The phytochemical analysis reveals the presence of steroids, fixed oils, flavonoids, glycosides, polysachharides and proteins. Ethanol extract showed significant wound healing effect by increasing wound contraction and significantly increase in hydroxyproline content of test group. The healing effect was compared with Povidone iodine ointment treated group of animals. Conclusion: In conclusion, the observation and results obtained in present study indicated that ethanol extract of Lens culinaris seeds was found improved skin healing may be due to presence of semi polar components like flavonoids.

Keywords: Lens culinaris, wound healing, povidone iodine, hydroxyproline, excision wound, flavonoids

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