Roshni Shivvedi1, 2, Satish Shilpi2, Shweta Kumar1, Amrita Singh1, Rajesh Singh Pawar1*
1Pharmacognosy and Phytochemistry Laboratory, VNS Group of Institutions, Bhopal, (M.P.)-462044, India
2Pharmaceutics Research Laboratory, Ravishankar College of Pharmacy, Bhopal (M.P.)-462037, India
*Address for Corresponding Author
Dr. Rajesh Singh Pawar
Truba Institute of Pharmaceutical Sciences, Bhopal (MP), India.
Former: Pharmacognosy and Phytochemistry Laboratory, VNS Group of Institutions, Bhopal, (M.P.)-462044, India.
Objective: This study was aimed to evaluate antioxidant activity of the Cannabis sativa extract (aqueous) using eukaryotic model Sachharomyces cerevisiae (both wild BY 4743 and knockout Δtrx2 Δctt Δcta) against H2O2 induced oxidative stress. Material and Methods: The antioxidant activity was investigated using viability assay, growth curve, spot assay and level of mitochondrial ROS determined by MitoSOX staining method. Results: The study showed the significant (**P<0.01) antioxidant activity of ACS. The treated yeast cells were able to maintain a higher level of growth as compared to the control. In viability assay the H2O2 stressed group showed 35% (wild) and 33% (Δtrx2) % viability of yeast cell while treated ACS 400µg/ml+H2O2 group showed improved % viability i.e. 50% (wild) and 57% (Δtrx2) after incubating for 30 minutes. In spot assay also ACS (400 µg/ml) showed a protective effect against challenge which is indicated by the growth in extract treated group. Compared to the negative control group the extract-treated group showed less fluorescence indicating the ability of the extract to scavenge mitochondrial superoxide and thereby protecting the cells. Conclusion: In this study, we found that aqueous extract of C. sativa exhibited marked free radical scavenging and antioxidant activity against hydrogen peroxide radicals in vivo. This extract can be used for the management of diseases like cancer, diabetes, neurodegenerative disease etc.
Keywords: Antioxidant, Hemp, Saccharomyces cerevisiae, ROS, oxidative stress