Research Articles

2017  |  Vol: 2(3)  |  Issue: 3 (May-June)
Basic treatment of bacterial cellulose for use in Regenerative Medicine

Jorge E. Rodriguez-Chanfrau1*, Márcio Luiz dos Santos1, Carla dos Santos Riccardi1, Gabriel Molina de Olyveira1, Pierre Basmaji2, Yaymarilis Veranes-Pantoja3, Antonio Carlos Guastaldi1

1Department of Physical Chemistry, Institute of Chemistry, Campus Araraquara, Paulista State University “Júlio de Mesquita Filho”, São Paulo, 14800-060, Brazil.

2Innovatec's - Biotechnology Research and Development, São Carlos-SP, 13560-042, Brazil

3Center of Biomaterials, University of Havana. Ave. Universidad s/n, e/ G y Ronda, Vedado, La Habana. CP 10400. Cuba

*Address for Corresponding Author

Jorge E. Rodríguez-Chanfrau

Department of Physical Chemistry,

Institute of Chemistry, Campus Araraquara, Paulista State University “Júlio de Mesquita Filho”, São Paulo, 14800-060, Brazil. 

Abstract

Objective: In this work, samples of bacterial cellulose tried with sodium hydroxide solution at 30% in water or isopropyl alcohol was characterized. Material and methods: The treated sample was analyzed using Thermogravimetric Analysis, Differential Scanning Calorimetry, X-ray Powder Diffraction studies, FTIR spectroscopy and Scanning Electron Microscopy. Results and conclusion: The results showed an increment in the solubility of the cellulose. The yield was superior to 85%. Thermogravimetric analysis showed that the treated sample with sodium hydroxide solution at 30% in isopropyl alcohol is more stable. The X-ray diffraction analysis showed a decrease in the intensities of the curves for both samples compared with untreated cellulose. The crystallinity index diminishes lightly and changes in the morphology of the cellulose after treated were observed. In conclusion, the results showed that the predominant region is crystalline after the treatment with sodium hydroxide.

Keywords: Bacterial cellulose, crystallinity index, Infrared spectroscopy, X-ray diffraction, Hydrogen Bands Intensity, Lateral Order Index 

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