Research Articles

2017  |  Vol: 2(3)  |  Issue: 3 (May-June)
Isolation and Screening of Protease Producing Soil Fungi

Arun Kumar Sharma1, Shreya Negi1, Vinay Sharma1*, Jyoti Saxena2

1Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, India.

2Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand, India

*Address for Corresponding Author:

Prof. Vinay Sharma

Head, Department of Bioscience & Biotechnology

Dean, Faculty of Science and Technology, Banasthali University-304022 (Rajasthan), India.

Abstract

Background: Proteases are enzymes, whose function is to cleave peptide bonds of proteins and split them down into small polypeptides or free amino acids. Fungi are commercially imperative organisms for their capability to secret enzymes including proteases into the surrounding environment. Proteases are currently used in various industries. Objectives: In the present investigation we have isolated and identified certain extracellular protease producing fungi among the other soil fungi. Materials and methods: Fungi were isolated by serial dilution method from soil samples collected from 3 different sites.  Isolated fungi were inoculated on skim milk, casein and gelatin agar plate for identification of extracellular protease producers. Results: A total of 31 fungal isolates were screened on agar medium. Among these, 6 isolates exhibited zone of hydrolysis on casein agar plates, 3 isolates exhibited clear zone on skim milk agar plates and 2 isolates were found positive on gelatin agar plates. Conclusion: Based on primary screening, we have identified 11 fungal isolates as protease producers. Proteolytic potential of these fungi can be further confirmed by secondary screening in submerged fermentation.

Keywords: Protease, fungi, soil, casein, gelatin, skim milk, agar plates

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