Research Articles

2020  |  Vol: 5(4)  |  Issue: 4 (July- August) | https://doi.org/10.31024/apj.2020.5.4.6
Preliminary pharmacological evaluation of Manilkara zapota stems bark extract for ulcerative colitis in rats

Anuj Kumar, Himanshu Bhushan Sahoo*

RKDF College of Pharmacy, SRK University, Hoshangabad road, Misrod, Bhopal - 462026 (MP) India

*Address for Corresponding Author

Dr. Himanshu Bhushan Sahoo

RKDF College of Pharmacy, SRK University, Hoshangabad road, Misrod, Bhopal - 462026 (MP) India

 

Abstract

Objective: The aim of present work was to investigate preliminary pharmacological screening of ethanol extract of Manilkara zapota stems barks for Dextran Sulphate Sodium (DSS) induced ulcerative colitis in rats. Material and methods: Phytochemical screening of Manilkara zapota stems barks (MZSB) extract was performed to detect the presence of different chemical constituents. Selected ethanol extract of MZSB was screened for DSS induced ulcerative colitis in rats. Group I was indicated untreated animals while Group II is control received DSS (3%w/v in drinking water) + 0.9% saline at a dose of 50 ml/kg. Treatment group III received DSS + ethanolic extract suspension 150 mg/kg and group IV received DSS + ethanolic extract suspension 200 mg/kg. Reference group received Sulfasalazine with 500 mg/kg suspension. The effect was observed by the measurement of disease activity Index and weight of colon along with MPO activity and MDA content. Antioxidants parameters were also observed to confirm the action behind protective effect of MZSB. Results and conclusion: Phytochemical study revealed that flavonoids and phenolic compounds are present in ethanolic extracts of Manilkara zapota ethanolic stem bark. The disease activity index and colon weight for different groups were observed. The disease activity index and weight of colon for observed significantly decreased in dose dependent manner. Results were also comparable to the standard drug treatment group. The ethanolic extract of Manilkara zapota stem bark (EEMZ) showed MPO activity significantly elevated whereas inhibited MPO activity with 200mg/kg as similar to standard drug.  The value of MDA content was decreased significantly similar to the standard group. Significant increase in GSH level and reduction in MDA level indicated antioxidant activity of EEMA. Hence, the probable mechanism of healing of ulcerative colitis of Manilkara zapota stem bark extract may be attributed to antioxidant and free radical scavenging effect may be attributed to presence of flavonoids and phenolic compounds.

Keywords: Manilkara zapota, Dextran Sulphate Sodium, Sulfasalazine, antioxidant, ulcerative colitis

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