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Isolation and purification of Glucose Oxidase from different Fungal sources Advance Pharmaceutical Journal

Review Articles

2016  |  Vol: 1(3)  |  Issue: 3 (July- August)
Isolation and purification of Glucose Oxidase from different Fungal sources

Aneeqa Tahir, Sikander Ali*

Institute of Industrial Biotechnology, Government College University, Lahore Pakistan

*Corresponding Author

Dr. Sikander Ali

Institute of Industrial Biotechnology,

Government College University, Lahore Pakistan


Objective: This paper discusses the main aspects of glucose oxidase. Glucose oxidase is an enzyme mainly obtained from filamentous fungi. Its most common source is A. niger which is easy to grow and extraction of GOD from it is relatively easy. Methods: Main mechanism of glucose oxidase is its property of being a flavor-protein which requires oxygen to form gluconic acid by the oxidation of sugar glucose. The composition of GOD is a dimeric glycoprotein with two similar polypeptide chains covalently linked with each other via di-sulphide linkages. In this review the characteristics and the analysis of glucose activity after its extraction are also discussed. Its extraction from different sources depends upon of few same and few different methods depending upon the organism it is obtained from. Conclusion: Glucose oxidase is a wonder enzyme having many functions and uses at the commercial level due to its significant properties. Many new technological advances are made to obtained pure, higher yield of glucose oxidase for various purposes.

Keywords: Glucose oxidase, filamentous fungus, enzyme purification, analysis, biosensors, yield


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