Research Articles

2020  |  Vol: 5(2)  |  Issue: 2(March-April) | https://doi.org/10.31024/apj.2020.5.2.5
Study of Rifampicin resistant pattern of Mycobacterium tuberculosis and Human immunodeficiency virus co-infected patients using Gene-Xpert assay

Abubakar Muhammad Inuwa1*, Abdulkadir Magaji Magashi1, Adam Uba Muhammad1,2, Umar Muazu Yunusa3

1Department of Microbiology Faculty of Life Sciences, Bayero University Kano, P.M.B 3011 Kano-Nigeria

2 Department of General Sciences, Aminu Dabo College of Health Sciences and Technology,Kano-Nigeria 

3Biochemistry Unit, Graduate School of Natural and Applied Sciences, Dokuz Eylul University, Izmir, Turkey

*Address for Correspondence Author

Abubakar Muhammad Inuwa

Department of Microbiology, Bayero University Kano-Nigeria P.M.B 3011

 

Abstract

Background: A major opportunistic infection among HIV-infected people is tuberculosis. It accelerates the deadly progression of HIV which results in a further decline in the patient's immune status and early death. Objective: The objective of the present study was to determine the rifampicin-resistant pattern of MTB and HIV co-infected patients. Material and methods: A total of 384 sputa and 384 blood samples were examined from 384 patients (247 male & 137 female) attending north-west zonal tuberculosis reference laboratory of Aminu Kano Teaching Hospital. The patients have a presumptive case of tuberculosis. The research was conducted between March and September 2019. The blood samples were first tested for HIV and sputum samples were analyzed using the GeneXpert machine, a real-time polymerase chain reaction-based equipment. Results: Eighty-six (52 male & 34 female) representing 22.4% of the participants are HIV positive. Likewise, 19 (12 male & 7 female) representing 4.9% of the total participants (384) has MTB. The highest rate of MTB was found within the age range of 31 - 40 years. Two (10.5%) samples from the male population are MTB positive and resistant to rifampicin. However, no rifampicin resistance was recorded in the female population. Age group 31 – 40 years are the most affected with pulmonary TB while age groups 21 - 30 and 30 - 40 years are the groups affected with rifampicin-resistant tuberculosis which was 0.38%. There was no detection of RIF/RES at >41 years of age group. Conclusion: MTB/RIF assay provide rifampicin resistance directly from the sputum in less than two hours.

Keywords: Mycobacterium tuberculosis, Human immunodeficiency virus, Rifampicin, Polymerase chain reaction, sputum

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