Research Articles

2018  |  Vol: 3(6)  |  Issue: 6 (November-December) | https://doi.org/10.31024/apj.2018.3.6.1
Antioxidant and anti-inflammatory activities of leaf extracts of Flacourtia jangomas (Lour.) Raeusch: An in vitro study

Senthil Kumar R.1,*, Vinoth Kumar S.1, Abdul Lathiff M.K.M.1, Uma Krithika S.1, Sudhakar P.2

1Department of Pharmaceutical Chemistry, Swamy Vivekanandha College of Pharmacy, Tiruchengode-637 205, Namakkal Dt., Tamilnadu, India

2Department of Pharmacology, Swamy Vivekanandha College of Pharmacy, Tiruchengode-637 205, Namakkal Dt., Tamilnadu, India

*Address for Corresponding Author

Dr. R. Senthil Kumar,

Professor & Head, Department of Pharmaceutical Chemistry,

Swamy Vivekanandha College of Pharmacy, Tiruchengode, Tamilnadu, India

Abstract

Objective: The present study aimed to evaluate the antioxidant and anti-inflammatory potential of ethanol and aqueous extract of F. jangomas using in vitro methods. Materials and Methods: Shade dried, pulverized leaves of F. jangomas was extracted with ethanol and water separately and subjected to qualitative and quantitative phytochemical analysis using standard procedures. The antioxidant potential of the plant extracts was assessed using ABTS, DPPH, hydroxyl radical, superoxide radical and hydrogen peroxide radical scavenging assay. The anti-inflammatory activity was assessed by nitric oxide inhibitory assay, 5-lipoxygenase inhibitory assay, cyclooxygenase inhibitory assay, HRBC membrane stability assay, Inhibition of protein denaturation and Proteinase inhibitory assay. Results: Qualitative phytochemical analysis indicated the presence of glycosides, alkaloids, phytosterols, tannins, flavonoids and phenolic compounds. The total phenol content of the ethanol and aqueous extracts was found to be 86.12 ± 2.41 & 74.38 ± 1.28mg of gallic acid equivalents per gram of dry extract, respectively. Total flavonoids content of ethanol extract was 168.14 ± 3.68 and in aqueous extract it was 145.42 ± 2.24mg of rutin equivalent per gram of dry extract.  Ethanol and aqueous extracts of F. jangomas potentially scavenge the free radicals in ABTS and DPPH radical scavenging assay methods. They exhibit moderate activity in hydroxyl, superoxide and hydrogen peroxide radical scavenging assays. The results obtained are comparable with ascorbic acid and rutin. In the in vitro anti-inflammatory assay, the extracts showed potent inhibition on the tested methods. Moreover, the extracts selectively block the action of cyclooxygenase-2 enzyme. Conclusion: These results demonstrated that the ethanol and aqueous extracts of F. jangomas possess antioxidant and anti-inflammatory effects and provide support of the traditional use of this plant in the treatment of inflammatory conditions. Further studies could reveal metabolites of the extracts responsible for the observed effects.

Keywords: Flacourtial jangomas, Free radical scavenging, Antioxidant, Anti-inflammatory, Cyclooxygenase, 5-lipoxygenase

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