Ankit Acharya*, Prakash Goudanavar, Vedamurthy Joshi
Department of Pharmaceutics, Sri Adichunchanagiri College of Pharmacy, B.G. Nagara-571448, Karnataka, India
*Address for Corresponding Author:
Mr. Ankit Acharya,
Department of Pharmaceutics,
Sri Adichunchanagiri College of Pharmacy, B.G. Nagar, Karnataka, India
Objective: The aim of the present study was to formulate and evaluate an ocular effective prolonged-release cubogel formulation of Timolol maleate for the treatment of glaucoma. Material and Methods: Timolol loaded cubosome was prepared by top down technique using glycerol monooleate and poloxamer 407 in different concentrations. Optimized batch of cubosome was selected based on the results of entrapment efficiency and in-vitro release results. Timolol maleate cubogel was prepared by dispersing cubosome in to in-situ gelling system. Interaction studies were confirmed by Fourier transforms infrared spectroscopy (FTIR) studies. The effect of surfactant and additives on particle size, entrapment efficiency and in-vitro drug release behaviour of cubosome was evaluated. Mucoadhesive strength was determined by ultracentrifugation and turbidity method. Optimized batch of cubogel was selected for short term stability studies. Results: FTIR results showed no sign of interaction drug and excipients, hence compatible. Formulation A6, CG1 and CG2 showed 78.84%, 54.77% and51.45% of drug release at the end of 12 hours and follow Higuchi’s release kinetic model, whereas marketed formulation completes its drug release within 2 hours. Cubogel formulation CG1 and CG2 exhibited a 2.51 and 2.376-fold increase in permeability (Papp) compared to marketed formulation, suggesting that higher amount of Timolol maleate-cubogel were taken up by the goat cornea than that of marketed eye drops. Conclusion: From the results obtained, it can be concluded that cubosome might a good alternative to conventional eye drops as it showed higher permeability and sustained release behaviour.
Keywords: Timolol maleate, cubosome, top down technique, permeability, glycerol monooleate, poloxamer 407