Research Articles

2020  |  Vol: 5(6)  |  Issue: 6 (November-December) | https://doi.org/10.31024/apj.2020.5.6.4
Proximate and phytochemical constituents of Ocimum sanctum in Sudan

Ahmed Ali Mustafa*, Hatil Hashim El-kamali

Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, P.O.Box#382, Omdurman, Sudan

*Address for Corresponding Author

Ahmed Ali Mustafa

Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, P.O.Box#382, Omdurman, Sudan

 


Abstract

 

Objective: In this study the proximate analysis and phytochemical constituents were carried out using standard reference methods. Material and methods: Moisture content, total ash, crude fiber and crude protein of Ocimum sanctum leaves and flowers were determined according to AOAC methods. The elemental analysis used Atomic Absorption Spectrometer (AAS) and flame emission for Ca, Fe, Na, K, Pb, Mn and Zn. Phytochemical screening of ethanolic extracts of Ocimum sanctum was performed for constituents e.g. carbohydrates, reducing sugars, monosacharides, proteins, amino acids, tannins, saponins, flavonoids, terpenoids and/or steroids, alkaloids, and antharaquinones. Results: The moisture content was found to be 3.68±0.03 flower and 6.51±0.01 leave, ash content 10.69±0.23 flower and 16.21±0.24 leave, crude fiber 35.73±0.05 flower and 10.74±0.06 leave and crude protein 10.64±0.05 flower and 19.39±0.13 leave. The elemental analysis used (AAS) and flame emission for Ca (75.01), Fe (2.826), Na (2.247), K (11.000), Pb (Not Detected), Mn (0.117) and Zn (0.205). Carbohydrates, reducing sugars, amino acids, tannins, saponins, terpenoids and/or steroids, alkaloids, antharaquinones were present in leaves and flowers. Conclusions: The results obtained showed was a good source of food and therapeutic.

Keywords: Ocimum sanctum, proximate analysis, phytochemical screening, antharaquinones

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