Ikpesu Thomas Ohwofasa1*, Opara Christina Ngozi2
1Department of Biology Federal University Otuoke, Nigeria
2Department of Microbiology, Federal University Otuoke Nigeria
Ikpesu, Thomas Ohwofasa
Department of Biology Federal University Otuoke, Nigeria
Cell number: +23480323121441
Objective: The in vitro antimicrobial screening of Solenostemon monostachyus and Ocimum gratissium against gram negative bacteria was investigated. Materials and methods: The plants were cultivated for this research, while Salmonella typhii, Escherichi coli and Eertrobacter aerogenes that were freshly isolated from clinical samples were studied. The leaves of both plants were analyzed quantitatively for the present of phytochemicals. The sensitivity of these bacteria isolates was performed using disk diffusion method using ofloxacin as a sensitivity drug. The antimicrobial activity was determined by measuring the diameter zones of inhibition (mm), and for the sensitive measurement (inhibitory zones ≥ 18) and resistant measurement (inhibitory zones ≤ 15). Results: The diameter zones of inhibition revealed that ethanol extract had the highest followed by methanol and aqueous extract. However, the synergy of the two plants extract had higher diameter zone of inhibition than individual plants extract. Conclusion: This study had shown the potency of the plants extract; therefore, more attention should be given to medicinal plants as they have shown to be clinically effective. Similarly, the plant’s extract can be used as research baseline to find out active natural products that may provide a leads in the formulation of new pharmaceuticals product to tackle resistance bacteria strains.
Keywords: Antimicrobial activity, Solenostemon monostachyus, Ocimum gratissium, Gram negative bacteria, ofloxacin