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Abubakar Dabo Dalhat1*, Dalha Wada Taura2, Dambazau Ado Musa2, Sadiya Suleiman Ayuba2, Musayyiba Shu’aibu2, Adam Uba Muhammad3
1Department of Biotechnology, Faculty of Life Science Mewar University, Gangrar, Chittorghar (RAJ) India
2Department of Microbiology Faculty of Life Science Bayero University Kano PMB: 3011.
3Department of General Science, Aminu Dabo College of Health Science and Technology, Kano, Nigeria
*Address for Corresponding Author:
Abubakar Dabo Dalhat
Department of Biotechnology, Mewar University,
Gangrar, Chittorghar (RAJ) India
Abstract
Objective: Aim of present study synergistic antibacterial effect of Mormodica charantia, Ocimum sanctum and Prosopis juliflora. Material and methods: Aqueous and methanol extracts of leaves of three important medicinal plant species, Mormodica charantia, Ocimum sanctum and Prosopis juliflora has been tested individually and in combination for their antibacterial activity against Staphylococcus aureus, Streptococcus pneumonia, Pseudomonas aeruginosa, Escherichia coli, klebsiella pneumoniae and Streptococcus pyogens using agar well diffusion method. Results and conclusion: Results showed higher antibacterial activity in combination of extracts of the medicinal plants studied. The methanolic leaf extracts of P. juliflora and aqueous leaf extracts of M. charantia showed 2.1 and 1.9 cm zone of inhibition (ZI), respectively against S. aureus and S. pyogens while tested individually. Whereas, the combination of methanolic leaf extracts of M. charantia + P. juliflora (1:2) and M. charantia + O. Sanctum (2:1) showed 2.3 cm ZI against S. aureus and S. Pyogens respectively. Similarly, the highest antibacterial activity of 2.5 cm ZI was observed against S. Pyogens in combination of methanolic leaf extracts (2:1:1) of all the three plants. This study clearly demonstrates the synergistic activity of plant extracts against different bacteria.
Keywords: Mormodica charantia, Ocimum sanctum, Prosopis juliflora, synergistic, zone of inhibition
