Government Pharmacy College, Government of Sikkim, Sikkim University, Sajong, Rumtek East Sikkim-737135
*Address of Corresponding Author
Mr. Sonam Bhutia, Assistant Professor
Department of Pharmacognosy, Government of Sikkim, Sajong, East Sikkim- 737135
Objective: Five ethnomedicinal plants from the Sikkim region, viz. Ocimum sanctum, Azadirachta indica, Trigonella foenum-graecum, Tinospora cordifolia and Gymnema sylvestre were considered in the present study. Materials and Methods: The present study will assist in standardization for quality, purity and sample identification. Here we mainly considered two standardization parameters like fluorescence activity and extraction yield respectively, the given medicinal plants were analysed using standard methods. Results: In the experimental analysis, we found that among extractive yield with different solvents, the aqueous extract displayed the highest yield followed respectively by ethanol. The fluorescence analysis for five Ethno-medicinal plants were conducted by using the visible light and ultra violet at 254nm and reveals the various colouration ranges from black to greenish brown colour with distinct morphological characteristics highlighted. Conclusion: The present study concludes that the data obtained can be used to authenticate, classify and standardize the above five ethno-medicinal plants. It was a first of its kind knowledge reported in a research paper.
Keywords: Fluorescence analysis, traditional plants, extractive values, Sikkim Himalaya, ethno-medicinal plants