Nishani J. Jayathilake1, Uthpala A. Jayewardena2
1Department of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Sri Lanka
2Department of Zoology, Faculty of Natural Sciences, The Open University of Sri Lanka, Sri Lanka
*Address for Correspondening Author
Nishani J. Jayathilake
Department of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Sri Lanka
Objective: Bohadschia vitiensis is historically proven for potential therapeutic values with anti-hypertension, anti-inflammatory, antimicrobial, antioxidant, antithrombotic, wound healing properties. Current study investigates the potential genotoxic effect of aqueous extract of Bohadschia vitiensis using Allium cepa genotoxicity model in order to validate the therapeutic usage. Materials and methods: Root grown onion bulbs were exposed to several concentrations of the extract; 62.5, 125, 250, 500 µg/ml, positive control (Dimethyl sulfoxide) and negative control (aged tap water) for 2 days. Immediately after the exposure period, root tips were harvested and observed in order to identify the existing stage of the cells and chromosomal aberrations. Results: The Mitotic Index was negatively correlated with the exposed concentrations and only the mitotic index of 500µg/ml was significantly different compared to the negative control. The extract showed no significant lethal genotoxic effect in comparison to the negative control within the tested range. Microscopic observations of the root tips exposed to the extract, revealed 0.1-0.2% of chromosomal aberrations including chromosomal bridges, chromosomal breaks, c-mitosis and vagrants. Conclusion: Moreover, the tested concentrations showed no significant effect on percentage chromosomal aberrations except 500µg/ml that showed only a sub lethal effect. Further, evaluation for toxicity with animal models is warranted for validating the toxicity of the extract prior to therapeutic applications.
Keyword: Genotoxicity; Allium cepa model, Sri Lankan Sea Cucumber species, Bohadschia vitiensis