Research Articles

2020  |  Vol: 5(5)  |  Issue: 5 (September- October) | https://doi.org/10.31024/apj.2020.5.5.3
Investigations on Canna indica rhizomes for nephroprotective activity with antioxidant effects

Preeti Singh, Himanshu Bhusan Sahoo*, Alok Pal Jain

RKDF College of Pharmacy, SRK University, Hoshangabad road, Misrod, Bhopal - 462026 (MP) India

*Address for Corresponding Author

Dr. Himanshu Bhusan Sahoo

RKDF College of Pharmacy, SRK University, Hoshangabad road, Misrod, Bhopal - 462026 (MP) India

 

Abstract

Objective: Present study was aimed for screening of Canna indica rhizomes for phytochemical investigation and nephroprotective activity on experimentally induced kidney toxicity. Material and methods: Animals were divided in 8 groups (n=6) and initial body weight was recorded. The group-I was administered vehicle for 15 days (p.o.). In II group, gentamicin (40 mg/kg b.w., s.c.) was administered for 15 days. The group III and IV were administered with ethanol extract of Canna indica rhizomes (EECA) (p.o.) at dose of 200 and 400 mg/kg, p.o, respectively. On 16th day, weight of animals was taken and blood samples were withdrawn through retro-orbital plexus. Changes occurring in body weight of animals along with other morphological changes were studied. Biochemical parameters during in vivo study were blood urea, serum creatinine and urine albumin studied. In addition, estimation of extent of lipid peroxidation and reduced glutathione (GSH) was also carried out to find out for observation of antioxidant effect of the extract. Results: In present study, significant increase in blood urea and creatinine has revealed toxicant effect of gentamicin on kidney/nephrons in negative control group. Gentamicin (40 mg/kg, s.c.) intoxicated group has shown significant increase in blood urea 83.61±3.62 mg/dL and creatinine levels 4.62±0.62mg/dL as compared to control group 31.82±1.27 mg/dL and 1.53±0.17 mg/dL, respectively. The ethanolic extract of Canna indica rhizomes at dose levels of 200 and 400 mg/kg have significantly protective effect in gentamicin induce nephrotoxicity. Results were also supported by histopathological studies in both preventive and curative groups. The significant improvement in GSH   level was observed after treatment with EECI (200 mg/kg and 400 mg/kg. Results were confirmed that DDPH scavenging effects were observed in dose dependent manner. GSH and MDA levels were supporting to antioxidant activity. Conclusion: In conclusion, ethanolic extract of Canna indica rhizomes have significant nephroprotective activities also supported with antioxidant activity. It is further concluded that this antioxidant activity is attributed to phenolic compounds, and flavonoids present in the extracts.

Keywords: Canna indica, nephroprotective, flavonoids, gentamicin, antioxidant

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