Jessa L. Carpio1, Perth Kenessa P. Ambida1,2
1Department of Biological Sciences, College of Science
2University Research and Development Services, University of Eastern Philippines, University Town, Northern Samar, Philippines 6400
*Address for Corresponding Author
Perth Kenessa P. Ambida
University Research and Development Services, University of Eastern Philippines, University Town, Northern Samar, Philippines 6400
Objective: Present study was aimed to determine antibacterial activity of Cassia alata Linn (Acapulco) roots and bark extract against Staphylococcus aureus and Escherichia coli. Material and methods: Two hundred fifty (250) grams of roots and three hundred (300) grams of bark were collected, washed and pounded in a pestle. The pounded mass was then filtered with a cheese cloth to extract the juice which was stored for future use. Commercially prepared antibacterial medicine (Chloramphenicol and Tetracycline) served as the positive control. Testing for the antibacterial activity of the crude extract was done using Whattman filter paper discs soaked in the test substances and impregnated into the plates streaked with S. aureus and E. coli. Observation for the presence or absence of an inhibition zone (clear area around the disc) was done after an incubation period of 24 hours at room temperature. Zones of inhibition were then measured with a plastic ruler. Results and conclusion: Results reveal that the root and bark crude extracts of Cassia alata (Linn) had antibacterial activity against the test organisms as indicated by the zones of inhibition around the filter and paper discs. Acapulco root crude extract had an average zone of inhibition of 0.3 mm on S. aureus and 3.0 mm on E. coli. The bark crude extract was more effective than the root extracts in as much as the former had a zone of inhibition of 1.3 mm on S. aureus and 6.3 mm on E. coli. Statistical analysis of the results show a significant difference on the activities of the root and bark crude extracts and the commercial antibiotic drugs. This implies that the extracts are not comparable in their effect on the test organisms with the commercial antibacterial drug.
Keywords: antibacterial, acapulco, S. aureus, E. coli, Cassia alata