Adam Uba Muhammad1*, Dalha Wada Taura1, Yunusa Umar Abubakar1, Abubakar Dabo Dalhat1, Abubakar Muhammad Inuwa1, Sadiq Magaji Aliyu1, Ramadan Muhammad Kabir2, Abubakar Khalil Rabil3
1Department of Microbiology Faculty of Life Sciences Bayero University Kano PMB: 3011
2Department of Microbiology Murtala Muhammad Specialist Hospital Kano-Nigeria.
3Department of Pure and Industrial Chemistry, Bayero University Kano
*Address for Corresponding Author
Adam Uba Muhammad
Department of Microbiology, Bayero University, Kano-Nigeria, P.M.B 3011
Background: It is well-known fact that medicinal plants are the resources of promising drugs for many ailments. Although, a number of research have been conducted on these plants, however, study related to their cytotoxic and antibacterial activity is of paramount important due to worldwide increase microbial resistance to antibiotics. Objective: The objective of present study was to determine the cytotoxicity and antibacterial activities of Cochlospermum tinctorium roots extract against some clinical isolates of bacteria. Material and Methods: Water and methanol were used in extraction of Cochlospermum tinctorium roots by percolation. The antibacterial activity was carried out using agar well diffusion technique against E. coli, Proteus specie, K. pneumoniae, P. aeruginosa and S. aureus, the minimum inhibitory concentration and the minimum bactericidal concentration were also determined using standard procedure The extracts were also subjected to Phytochemical Screening, Brine-shrimp lethality assay, the Lc 50 values were determined by linear regression analysis method. Results: The result of phytochemical screening revealed the presence of secondary metabolites, the result of antibacterial activity ranged from 7.23±0.20mm to 32.67±0.58mm inhibition zones, N-hexane extract have the highest zone of inhibition (32.67±0.58mm) against K. pneumoniae at 2000μg/ml concentrations, the extract was active on all the tested microorganisms at different concentrations, the MIC was lowest for K. pneumoniae at (62.5µg /ml) and highest for S. aureus and E. coli at (125µg/ml), the MBC result showed that, the extract was bactericidal to K. pneumoniae at (62.5 µg/ml followed by S. aureus at (125µg/ml) and E. coli at (250µg/ml), the cytotoxicity of the plant extract (LC50) showed that, the plant was weakly toxic. Conclusion: These findings therefore support the local use of C. tinctorium root extracts for the treatment of bacterial infections in Northern Nigeria.
Keywords: Brine shrimp lethality assay, Cochlospermum tinctorium, cytotoxic, linear regression analysis, antibacterial