Udeme Owunari Georgewill1*, Elias Adikwu2
1Department of Pharmacology, Faculty of Basic Clinical Sciences, University of Port Harcourt, Rivers State, Nigeria
2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Niger Delta University, Bayelsa State, Nigeria
*Address for Corresponding Author
Dr. Udeme Owunari Georgewill
Department of Pharmacology, Faculty of Basic Clinical Sciences, University of Port Harcourt, Rivers State, Nigeria
Objective: Antimalarial drug resistance is one of the greatest challenges toward eradicating malaria. Exploring new combination therapies can overcome resistance challenges. The present study examined the antiplasmodial effect of artemether/lumefantrine/doxycycline (A/L/D) on a mouse model infected with Plasmodium berghei (P. berghei). Materials and Methods: Swiss albino mice (22-25g) intraperitoneally infected with blood containing 1x107P. berghei were randomly grouped and orally treated with D (2.2 mg/kg), A/L (2.3/13.7 mg/kg)and A/L/D. The negative and the positive controls were treated with normal saline (0.2ml) and chloroquine (CQ) (10mg/kg) daily for 4 days, respectively. After treatment, blood samples were assessed for percentage parasitemia, biochemical parameters. The mice were also observed for mean survival time (MST). Results and Conclusion: In the curative, suppressive and prophylactic tests, D, A/Land A/L/D decreased percentage parasitemia levels at p<0.05; p<0.01 and p<0.001, respectively when compared to negative control. In the curative test, D, A/L, A/L/D and CQ produced 60.4%, 70.0%, 81.2% and 76.0% parasitemia inhibitions, respectively. MST was prolonged by D, A/L, and A/L/D at p<0.05; p<0.01 and p<0.001 respectively. D, A/L, and A/L/D prevented P. berghei-induced alterations in biochemical parameters by increasing packed cell volume, red blood cells, hemoglobin, and high-density lipoprotein cholesterol and decreasing white blood cells, cholesterol, total cholesterol, low-density lipoprotein cholesterol, and triglyceride levels significantly at (p<0.05) and (p<0.01) and (p<0.001) when compared to negative control. A/L/D produced significant antiplasmodial activity therefore; it may be used clinically for the treatment of malaria.
Keywords: Antiplasmodial, artemether tetracycline, antimalarial, lumefantrine, Plasmodium berghei