Research Articles

2020  |  Vol: 5(5)  |  Issue: 5 (September- October) | https://doi.org/10.31024/apj.2020.5.5.2
In vitro antibacterial activity of Cannabis sativa (Hemp) extracts against avian pathogenic Escherichia coli (APEC) isolated from Broilers Chicken

Yunusa Umar Abubakar1*, Dalha Wada Taura2, Binta Aminu Muhammad2, Tukur Shehu Ibrahim1, Abdulhamed Baba Njobdi1, Muhammed Umar1, Abdulhamed Baba Gjobdi1Patience O. Ihedigbo1, Miracle Uwa Livinus2

1Department of Animal Health College of Agriculture Jalingo, Taraba State, Nigeria

2Department of Microbiology, Bayero University, Kano, Nigeria

*Address for Corresponding author

Department of Animal Health College of Agriculture Jalingo, Taraba State, Nigeria

 

Abstract

Objective: To investigate the antibacterial activity of ethyl acetate and methanol extracts of Cannabis sativa (C. sativa) on avian pathogen. Material and methods: Avian Pathogenic Escherichia coli (APEC) was isolated from 200 diseased broilers chicken. The heart swab and faecal samples of the naturally infected broilers chicken were collected from poultry farm of College of Agriculture, Jalingo, Taraba State and the associated avian bacteria was isolated and identified through cultural and biochemical methods. The seed and leaves powder were extracted successively with 500 ml of ethyl acetate and methanol solvents by using Soxhlet extractor for 8hr at a temperature not exceeding the boiling point of the solvent. Ethyl acetate and methanol extracts were subjected to phytochemical analysis as well as the antimicrobial assay using agar well diffusion method. Results and conclusion: The yields of the extracts were in the order; ELE (48.5%) > MLE (28%) > MSE (14.4%) > ESE (7.8%). Ethyl-acetate seed and leave extracts showed the highest antibacterial activity of 21.50±0.71mm and 21.00±1.41mm respectively against E. coli at 2000µg/ml. The findings also revealed that the antibacterial activities of C. sativa plant extracts were in the following order; ESE>ELE>MSE>MLE. The present study revealed that the extracts C. sativa seed and leaves possess significant antibacterial activity on isolated avian pathogen. To conclude the present findings, the ethyl acetate seed and leaf extracts could be used as potential sources of antibacterial agents.

Keywords: Avian, pathogen, poultry, Cannabis sativa, phytochemical, antibacterial

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