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Anil Kumar Pandey, Alok Pal Jain, Anjana Bhardwaj*
RKDF College of Pharmacy, SRK University, Hoshangabad Road, Misrod, Bhopal - 462026 (MP) India
*Address for Corresponding Author
Prof. Anjana Bhardwaj
RKDF College of Pharmacy, SRK University, Hoshangabad road, Misrod, Bhopal - 462026 (MP) India
Abstract
Background: Ulcerations and inflammation occur in ulcerative colitis in the inner lining of the colon, resulting in symptoms of abdominal pain, diarrhoea and rectal bleeding. Objective: Aim of present study was to prepare and characterized luteolin loaded microspheres for effective treatment of ulcerative colitis. Materials and methods: Pre-formulation studies of luteolin were performed to characterize the drug and then Luteolin microsphere was prepared by solvent evaporation method through different optimized parameters. For characterization of prepared microspheres various parameters like particle size, drug entrapment, percentage yield, shape and surface properties were analysed. Scanning electron microscopy of optimized formulation F1 was confirmed the spherical shape of microspheres. The in vitro stability of luteolin microspheres were stored at 5±3ºC, 25±2ºC/60±5% RH and 40±2ºC /75±5% RH. Changes in particle size after time interval of 30, 60 and 90 days were determined. DSS produces severe macroscopic edematous inflammation in the colon. The disease activity index and wet colon weight for different groups were observed in DSS induced ulcerative colitis. Results and conclusion: The disease activity index and weight of colon for colitis control group were found as 4.12±0.82, 201.72±4.37, respectively. The disease activity index and colon weight for LUT and LUT microsphere treated groups of animals were observed significantly decreased as 2.63±0.27 and 1.53±0.62. LUT Microsphere decreases weight of colon significantly as 133.96±3.21. In conclusion, the free radical scavenging property of Luteolin plays a significant role in ulcer healing. Significant increase in GSH level and reduction in MDA level has also been revealed in extracts treated groups while investigating in vivo antioxidant activity.
Keywords: Luteolin, ulcerative colitis, antioxidant, inflammation, microsphere, dextran sodium sulfate
